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KEY RESOURCES TABLE
Dynamo Flash Sybr Green Qpcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynamo colorflash sybr green qpcr mix  (Thermo Fisher)
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KEY RESOURCES TABLE
Dynamo Colorflash Sybr Green Qpcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynamo flash sybr green qpcr master mix  (Thermo Fisher)
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flt1 deletion causes the upregulation of endothelial MAPK/ERK signaling and the downregulation of egr3 expression during cardiac regeneration. (A,B) Heat-map (A) and <t>RT-qPCR</t> validation (B) showing differentially regulated genes of interest in the border zone and injured area (BZI) of cryoinjured ventricles from flt1 +/+ and flt1 −/− zebrafish at 96 hpci. (C,C′) Immunostaining of cryoinjured ventricles from flt1 +/+ ( n =4, C) and flt1 −/− ( n =4, C′) zebrafish at 96 hpci for pERK (phosphorylated ERK, white). Red lines indicate the range of pERK + injured area. Dotted orange line indicates the injured area. (D) Quantification of the percentage of pERK + injured area in the indicated genotypes at 96 hpci. (E,E′) Immunostaining for pERK (green) and EGFP (cECs, magenta) with DAPI (blue) counterstaining on sections of cryoinjured ventricles from Tg(flt1:Mmu.Fos-EGFP);flt1 +/+ ( n =4, E) and Tg(flt1:Mmu.Fos-EGFP);flt1 −/− ( n =6, E′) zebrafish at 96 hpci. Arrowheads indicate pERK + cECs in the BZI. Areas outlined are shown at higher magnification on the right. (F,G) Quantification showing the number (F) and percentage (G) of pERK + cECs in the BZI of the indicated genotypes at 96 hpci. (H,I) Immunostaining for EGFP (green), MHC (myosin heavy chain, magenta) and Aldh1a2 (red) with DAPI (blue) counterstaining on representative sections from untouched (H) and cryoinjured (I) Pt(egr3:Gal4-VP16);Tg(5xUAS:EGFP) (abbreviated as egr3>EGFP ) ventricles at 96 hpci. Arrowheads indicate EGFP + cells. Areas outlined are shown at higher magnification on the right. White dotted lines indicate the injured area. epi, epicardium. Scale bars: 100 µm. Data are mean±s.e.m. (two-tailed, unpaired Student's t -test with P values shown in the graphs).
Dynamo Flash Sybr Green Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynamo color flash sybr green rt-qpcr master mix  (Thermo Fisher)
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flt1 deletion causes the upregulation of endothelial MAPK/ERK signaling and the downregulation of egr3 expression during cardiac regeneration. (A,B) Heat-map (A) and <t>RT-qPCR</t> validation (B) showing differentially regulated genes of interest in the border zone and injured area (BZI) of cryoinjured ventricles from flt1 +/+ and flt1 −/− zebrafish at 96 hpci. (C,C′) Immunostaining of cryoinjured ventricles from flt1 +/+ ( n =4, C) and flt1 −/− ( n =4, C′) zebrafish at 96 hpci for pERK (phosphorylated ERK, white). Red lines indicate the range of pERK + injured area. Dotted orange line indicates the injured area. (D) Quantification of the percentage of pERK + injured area in the indicated genotypes at 96 hpci. (E,E′) Immunostaining for pERK (green) and EGFP (cECs, magenta) with DAPI (blue) counterstaining on sections of cryoinjured ventricles from Tg(flt1:Mmu.Fos-EGFP);flt1 +/+ ( n =4, E) and Tg(flt1:Mmu.Fos-EGFP);flt1 −/− ( n =6, E′) zebrafish at 96 hpci. Arrowheads indicate pERK + cECs in the BZI. Areas outlined are shown at higher magnification on the right. (F,G) Quantification showing the number (F) and percentage (G) of pERK + cECs in the BZI of the indicated genotypes at 96 hpci. (H,I) Immunostaining for EGFP (green), MHC (myosin heavy chain, magenta) and Aldh1a2 (red) with DAPI (blue) counterstaining on representative sections from untouched (H) and cryoinjured (I) Pt(egr3:Gal4-VP16);Tg(5xUAS:EGFP) (abbreviated as egr3>EGFP ) ventricles at 96 hpci. Arrowheads indicate EGFP + cells. Areas outlined are shown at higher magnification on the right. White dotted lines indicate the injured area. epi, epicardium. Scale bars: 100 µm. Data are mean±s.e.m. (two-tailed, unpaired Student's t -test with P values shown in the graphs).
Dynamo Color Flash Sybr Green Rt Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Microglial APOE3 Christchurch protects neurons from Tau pathology in a human iPSC-based model of Alzheimer’s disease

doi: 10.1016/j.celrep.2024.114982

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DyNAmo Flash SYBR Green qPCR mix , Thermo Fisher Scientific , Cat # F416.

Techniques: Marker, Recombinant, SYBR Green Assay, Knock-In, Software

flt1 deletion causes the upregulation of endothelial MAPK/ERK signaling and the downregulation of egr3 expression during cardiac regeneration. (A,B) Heat-map (A) and RT-qPCR validation (B) showing differentially regulated genes of interest in the border zone and injured area (BZI) of cryoinjured ventricles from flt1 +/+ and flt1 −/− zebrafish at 96 hpci. (C,C′) Immunostaining of cryoinjured ventricles from flt1 +/+ ( n =4, C) and flt1 −/− ( n =4, C′) zebrafish at 96 hpci for pERK (phosphorylated ERK, white). Red lines indicate the range of pERK + injured area. Dotted orange line indicates the injured area. (D) Quantification of the percentage of pERK + injured area in the indicated genotypes at 96 hpci. (E,E′) Immunostaining for pERK (green) and EGFP (cECs, magenta) with DAPI (blue) counterstaining on sections of cryoinjured ventricles from Tg(flt1:Mmu.Fos-EGFP);flt1 +/+ ( n =4, E) and Tg(flt1:Mmu.Fos-EGFP);flt1 −/− ( n =6, E′) zebrafish at 96 hpci. Arrowheads indicate pERK + cECs in the BZI. Areas outlined are shown at higher magnification on the right. (F,G) Quantification showing the number (F) and percentage (G) of pERK + cECs in the BZI of the indicated genotypes at 96 hpci. (H,I) Immunostaining for EGFP (green), MHC (myosin heavy chain, magenta) and Aldh1a2 (red) with DAPI (blue) counterstaining on representative sections from untouched (H) and cryoinjured (I) Pt(egr3:Gal4-VP16);Tg(5xUAS:EGFP) (abbreviated as egr3>EGFP ) ventricles at 96 hpci. Arrowheads indicate EGFP + cells. Areas outlined are shown at higher magnification on the right. White dotted lines indicate the injured area. epi, epicardium. Scale bars: 100 µm. Data are mean±s.e.m. (two-tailed, unpaired Student's t -test with P values shown in the graphs).

Journal: Development (Cambridge, England)

Article Title: flt1 inactivation promotes zebrafish cardiac regeneration by enhancing endothelial activity and limiting the fibrotic response

doi: 10.1242/dev.203028

Figure Lengend Snippet: flt1 deletion causes the upregulation of endothelial MAPK/ERK signaling and the downregulation of egr3 expression during cardiac regeneration. (A,B) Heat-map (A) and RT-qPCR validation (B) showing differentially regulated genes of interest in the border zone and injured area (BZI) of cryoinjured ventricles from flt1 +/+ and flt1 −/− zebrafish at 96 hpci. (C,C′) Immunostaining of cryoinjured ventricles from flt1 +/+ ( n =4, C) and flt1 −/− ( n =4, C′) zebrafish at 96 hpci for pERK (phosphorylated ERK, white). Red lines indicate the range of pERK + injured area. Dotted orange line indicates the injured area. (D) Quantification of the percentage of pERK + injured area in the indicated genotypes at 96 hpci. (E,E′) Immunostaining for pERK (green) and EGFP (cECs, magenta) with DAPI (blue) counterstaining on sections of cryoinjured ventricles from Tg(flt1:Mmu.Fos-EGFP);flt1 +/+ ( n =4, E) and Tg(flt1:Mmu.Fos-EGFP);flt1 −/− ( n =6, E′) zebrafish at 96 hpci. Arrowheads indicate pERK + cECs in the BZI. Areas outlined are shown at higher magnification on the right. (F,G) Quantification showing the number (F) and percentage (G) of pERK + cECs in the BZI of the indicated genotypes at 96 hpci. (H,I) Immunostaining for EGFP (green), MHC (myosin heavy chain, magenta) and Aldh1a2 (red) with DAPI (blue) counterstaining on representative sections from untouched (H) and cryoinjured (I) Pt(egr3:Gal4-VP16);Tg(5xUAS:EGFP) (abbreviated as egr3>EGFP ) ventricles at 96 hpci. Arrowheads indicate EGFP + cells. Areas outlined are shown at higher magnification on the right. White dotted lines indicate the injured area. epi, epicardium. Scale bars: 100 µm. Data are mean±s.e.m. (two-tailed, unpaired Student's t -test with P values shown in the graphs).

Article Snippet: RT-qPCR reactions were performed using the DyNAmo Flash SYBR Green qPCR master mix (Thermo Fischer Scientific) on a CFX Connect Real-Time BioRad machine. mRNA levels were normalized against the housekeeping gene rpl13 .

Techniques: Expressing, Quantitative RT-PCR, Biomarker Discovery, Immunostaining, Two Tailed Test